Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
Quick Facts
- Publication title: Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles
- Journal: Biol Proced Online
- Year: 2020
- DOI: 10.1186/s12575-020-00125-5
- PMID/PMCID: 32587481; PMC7313174
Research overview
Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. Combining SEC with UC could significantly improve the performance of mass spectrometry-based proteomic profiling in analyzing plasma-derived sEVs.
Key findings
Plasma samples were collected from healthy individuals, and sEVs were separated by one-step SEC, one-step UC, and combining SEC with UC, respectively.
Here we exhibited that the purity of sEVs was improved by SEC in contrast to traditional UC.
Furthermore, by conducting a SEC procedure followed by UC, we separated sEVs with the highest purity.
In the proteomic analysis, 992 protein species were identified in the plasma sEVs isolated by our novel separation method, of which several proteins are sEVs-associated proteins but hitherto never been identified in the previous studies and database, much more than plasma sEVs isolated by UC (453) or SEC (682) alone.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Combination of Size-Exclusion Chromatography and Ultracentrifugation Improves the Proteomic Profiling of Plasma-Derived Small Extracellular Vesicles Biol Proced Online. 2020. DOI: 10.1186/s12575-020-00125-5. PMID/PMCID: 32587481; PMC7313174.