Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis

Quick Facts

  • Publication title: Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis
  • Journal: INT J NANOMED
  • Year: 2021
  • DOI: 10.2147/IJN.S336062
  • PMID/PMCID: 34938076; PMC8687685

Research overview

This study aimed to compare the therapeutic effects of two agents on osteoarthritis (OA) and investigate underlying mechanism using proteomics. These data suggest the promising potential of hUC-MSC-sEVs as a therapeutic agent for OA.

Key findings

In vitro data showed that hUC-MSC-sEVs were taken up by chondrocytes.

A total of 15 μg/mL of sEVs show the greatest proliferative and migratory capacities among all groups.

In the animal study, hUC-MSCs and hUC-MSC-sEVs alleviated cartilage damage.

This effect was mediated via maintaining cartilage homeostasis, as was confirmed by upregulation of the COL II and downregulation of the MMP13 and ADAMTS5.

Study design

In vitro, the proliferation and migration abilities of chondrocytes treated with hUC-MSCs or hUC-MSC-sEVs were detected by Cell Counting Kit-8 assay and scratch wound assay.

In vivo, hUC-MSCs (a single dose of 5 × 105) or hUC-MSC-sEVs (30 μg/time) were injected into the knee joints of anterior cruciate ligament transection-induced OA model.

Hematoxylin and eosin, Safranin O/Fast Green staining were used to observe cartilage degeneration.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Comparison of Curative Effect of Human Umbilical Cord-Derived Mesenchymal Stem Cells and Their Small Extracellular Vesicles in Treating Osteoarthritis INT J NANOMED. 2021. DOI: 10.2147/IJN.S336062. PMID/PMCID: 34938076; PMC8687685.