A comprehensive evaluation of stability and safety for HEK293F-derived extracellular vesicles as promising drug delivery vehicles

Quick Facts

  • Publication title: A comprehensive evaluation of stability and safety for HEK293F-derived extracellular vesicles as promising drug delivery vehicles
  • Journal: J Control Release
  • Year: 2025
  • DOI: 10.1016/j.jconrel.2025.113673
  • PMID/PMCID: 40169120

Research overview

Our study demonstrates that HEK293F-EVs maintain stable physicochemical characteristics and compositions across batches and possess a superior safety profile, suggesting their significant potential as a safe and reliable drug delivery platform for clinical applications. A comprehensive understanding of their batch stability and in vivo safety is prerequisite for clinical translation.

Key findings

HEK293F-derived extracellular vesicles (HEK293F-EVs) have great potential as next-generation drug delivery vehicles.

A comprehensive understanding of their batch stability and in vivo safety is prerequisite for clinical translation.

HEK293F-EVs were purified using ultracentrifugation combined with size exclusion chromatography, and their physicochemical properties, such as morphology, size distribution, and biomarkers, were thoroughly characterized.

Raman spectroscopy and multi-omics analyses were employed to elaborate their molecular composition.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV proteomics; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Cell-Culture Supernatant EV Isolation & Purification, EV Re-purification / Free-Dye Removal, EV Proteomics, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: A comprehensive evaluation of stability and safety for HEK293F-derived extracellular vesicles as promising drug delivery vehicles Journal of controlled release. 2025. DOI: 10.1016/j.jconrel.2025.113673. PMID/PMCID: 40169120.