A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues
Quick Facts
- Publication title: A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues
- Journal: Biotechniques
- Year: 2022
- DOI: 10.2144/btn-2022-0035
- PMID/PMCID: 35946315
Research overview
Extracellular vesicles (EVs) are small vesicles mediating intercellular communications that have been widely used in disease diagnosis. Extracting EVs from tissues is of great importance, but current approaches are finite and the EV yield is limited.
Key findings
Extracellular vesicles (EVs) are small vesicles mediating intercellular communications that have been widely used in disease diagnosis.
Extracting EVs from tissues is of great importance, but current approaches are finite and the EV yield is limited.
Here, the authors introduced a new method to increase EV yield based on frozen sectioning.
With a standardized, semiautomated tissue-slicing procedure in a cryostat, the authors successfully isolated EVs from hearts, kidneys and stomachs.
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References
Original publication: A cryostat-based frozen section method to increase the yield of extracellular vesicles extracted from different tissues Biotechniques. 2022. DOI: 10.2144/btn-2022-0035. PMID/PMCID: 35946315.