Delivering miR-23b-3p by small extracellular vesicles to promote cell senescence and aberrant lipid metabolism

Quick Facts

  • Publication title: Delivering miR-23b-3p by small extracellular vesicles to promote cell senescence and aberrant lipid metabolism
  • Journal: BMC Biol
  • Year: 2025
  • DOI: 10.1186/s12915-025-02143-9
  • PMID/PMCID: 39934790; PMC11817603

Research overview

In the present study, we extracted liver tissue small extracellular vesicles (sEVs) of mice at different ages and performed transcriptome and proteome analyses to investigate the senescence-associated secretory phenotype (SASP) and mechanisms. Targeting miR-23b-3p could provide new therapeutic strategies for ameliorating age-related liver dysfunction and associated metabolic abnormalities.

Key findings

In the present study, we extracted liver tissue small extracellular vesicles (sEVs) of mice at different ages and performed transcriptome and proteome analyses to investigate the senescence-associated secretory phenotype (SASP) and mechanisms.

sEVs in the older group were rich in miR-23b-3p, which was abundant in the sEVs of induced aging cells and promoted cell senescence by targeting TNF alpha induced protein 3 (Tnfaip3).

After injecting adeno-associated virus (AAV) expressing miR-23b-3p into mice, the liver of mice in the experimental group displayed a more evident inflammatory response than that in the control group.

Additionally, we found elevated miR-23b-3p in blood-derived-sEVs from patients with familial hypercholesterolemia.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, EV proteomics, qPCR validation; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics, qPCR Validation, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Delivering miR-23b-3p by small extracellular vesicles to promote cell senescence and aberrant lipid metabolism BMC biology. 2025. DOI: 10.1186/s12915-025-02143-9. PMID/PMCID: 39934790; PMC11817603.