ECM stiffness affects cargo sorting into MSC-EVs to regulate their secretion and uptake behaviors

Quick Facts

  • Publication title: ECM stiffness affects cargo sorting into MSC-EVs to regulate their secretion and uptake behaviors
  • Journal: J NANOBIOTECHNOL
  • Year: 2024
  • DOI: 10.1186/s12951-024-02411-w
  • PMID/PMCID: 38515095; PMC10956366

Research overview

Using multi-omics analysis, we found that a decrease in ECM stiffness impeded the sorting of vesicular transport-related proteins and autophagy-related lipids into MSC-EVs, impairing their secretion and subsequent uptake by macrophages. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have garnered extensive attention as natural product-based nanomedicines and potential drug delivery vehicles.

Key findings

Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have garnered extensive attention as natural product-based nanomedicines and potential drug delivery vehicles.

However, the specific mechanism for regulating MSC-EVs secretion and delivery remains unclear.

Here, we demonstrate that extracellular matrix (ECM) stiffness regulates the secretion and delivery of EVs by affecting MSCs' cargo sorting mechanically.

Using multi-omics analysis, we found that a decrease in ECM stiffness impeded the sorting of vesicular transport-related proteins and autophagy-related lipids into MSC-EVs, impairing their secretion and subsequent uptake by macrophages.

Echo Biotech Role

Echo Biotech contributed miRNA profiling/sequencing and bioinformatic analysis.

Related platforms: Exoomics®

Related services and capabilities: EV miRNA Profiling / Sequencing

References

Original publication: ECM stiffness affects cargo sorting into MSC-EVs to regulate their secretion and uptake behaviors Journal of nanobiotechnology. 2024. DOI: 10.1186/s12951-024-02411-w. PMID/PMCID: 38515095; PMC10956366.