Effects of lysate/tissue storage at -80° C on subsequently extracted EVs of epithelial ovarian cancer tissue origins
Quick Facts
- Publication title: Effects of lysate/tissue storage at -80° C on subsequently extracted EVs of epithelial ovarian cancer tissue origins
- Journal: iScience
- Year: 2023
- DOI: 10.1016/j.isci.2023.106521
- PMID/PMCID: 37123245; PMC10139970
Research overview
Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication. Cryopreserved EOC tissue may benefit clinical applications for studies of tissue-derived EVs, especially EV proteins-related ones.
Key findings
Small extracellular vesicles (sEVs) and large extracellular vesicles (lEVs), play vital roles in intercellular communication.
We optimized a method that extracts EVs from epithelial ovarian cancer (EOC) tissues for the purpose of investigating whether cryopreservation of EOC tissues affects the phenotypes, contents, and biological functions of extracted EVs.
EV morphology, the number and size distribution of EVs, and EV-related markers were analyzed.
Storage of lysates at -80°C decreased lEV yield and increased sEV yield, whereas storage of tissues at -80°C increased both sEV and lEV yields; neither changed the morphology or particle mass ratio of EVs.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, EV proteomics.
Related platforms: Exoomics®
Related services and capabilities: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics
References
Original publication: Effects of lysate/tissue storage at -80° C on subsequently extracted EVs of epithelial ovarian cancer tissue origins iScience. 2023. DOI: 10.1016/j.isci.2023.106521. PMID/PMCID: 37123245; PMC10139970.