Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs
Quick Facts
- Publication title: Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs
- Journal: J Extracell Vesicles
- Year: 2019
- DOI: 10.1080/20013078.2019.1643670
- PMID/PMCID: 31448068; PMC6691764
Research overview
Early diagnosis of colon cancer (CC) is clinically important, as it can significantly improve patients' survival rate and quality of life. In conclusion, we suggested that circulating sEVs enriched fractions have a distinct miRNA profile in CC patients, and sEVs derived miRNA could be used as a promising biomarker to detect CC at an early stage.
Key findings
Early diagnosis of colon cancer (CC) is clinically important, as it can significantly improve patients' survival rate and quality of life.
Although the potential role for small extracellular vesicles (sEVs) in early detection of many diseases has been repeatedly mentioned, systematic screening of plasma sEVs derived early CC specific biomarkers has not yet been reported.
In this work, plasma sEVs enriched fractions were derived from 15 early-stage (TisN0M0) CC patients and 10 normal controls (NC).
RNA sequencing identified a total number of 95 sEVs enriched fraction derived miRNAs with differential expression between CC and NC, most of which (60/95) was in well accordance with tissue results in the Cancer Genome Atlas (TCGA) dataset.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, qPCR validation.
Related platforms: Exoomics®
Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation
References
Original publication: Evaluation of circulating small extracellular vesicles derived miRNAs as biomarkers of early colon cancer: a comparison with plasma total miRNAs J Extracell Vesicles. 2019. DOI: 10.1080/20013078.2019.1643670. PMID/PMCID: 31448068; PMC6691764.