Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma
Quick Facts
- Publication title: Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma
- Journal: Endocrine
- Year: 2021
- DOI: 10.1007/s12020-021-02949-x
- PMID/PMCID: 34854020
Research overview
In the present study, we aimed to identify whether plasma exosomal miRNAs could be potential diagnostic markers of LNM in PTC. The two plasma exosomal miRNAs (miR-6774-3p and miR-6879-5p) and their combination could serve as new promising biomarkers for the diagnosis of LNM in PTC patients.
Key findings
In total, 197 miRNAs were found to be differentially expressed in the testing set.
001) in the PTC-N1 patients was significantly higher than that in the controls.
962) were higher than those of the total miRNAs directly isolated from plasma.
Moreover, the expression of exosomal miRNAs was stable after treatment with RNase A, prolonged incubation, or repeated freezing and thawing.
Study design
Profiles of plasma exosomal miRNAs were screened using miRNA microarrays.
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was performed in the validation and diagnostic sets to select candidate exosomal miRNAs.
Finally, receiver operating characteristic (ROC) curves were generated to evaluate the efficiency of target exosomal miRNAs in distinguishing PTC-N1 patients from PTC-N0 patients.
Echo Biotech Role
Echo Biotech contributed EV characterization.
Related platforms: Exoomics®
Related services and capabilities: EV Basic Characterization (TEM/NTA/WB)
References
Original publication: Evaluation of plasma exosomal miRNAs as potential diagnostic biomarkers of lymph node metastasis in papillary thyroid carcinoma Endocrine. 2021. DOI: 10.1007/s12020-021-02949-x. PMID/PMCID: 34854020.