Exosome-based WTAP siRNA delivery ameliorates myocardial ischemia-reperfusion injury

Quick Facts

  • Publication title: Exosome-based WTAP siRNA delivery ameliorates myocardial ischemia-reperfusion injury
  • Journal: Eur J Pharm Biopharm
  • Year: 2024
  • DOI: 10.1016/j.ejpb.2024.114218
  • PMID/PMCID: 38367759

Research overview

In the present study, we observed the up-regulation of the m6A mRNA methylation complex component Wilms' tumor 1-associating protein (WTAP) in MI/R mice, which led to the m6A modification of TXNIP mRNA and an increase in mRNA abundance. The increased expression of Thioredoxin-interacting protein (TXNIP) has been implicated in MI/R injury, although the detailed mechanism remains incompletely understood.

Key findings

Myocardial ischemia/reperfusion (MI/R) injury is the primary cause of postischemicheartfailure.

The increased expression of Thioredoxin-interacting protein (TXNIP) has been implicated in MI/R injury, although the detailed mechanism remains incompletely understood.

In the present study, we observed the up-regulation of the m6A mRNA methylation complex component Wilms' tumor 1-associating protein (WTAP) in MI/R mice, which led to the m6A modification of TXNIP mRNA and an increase in mRNA abundance.

Knock-down of WTAP resulted in a significant reduction in the m6A level of TXNIP mRNA and down-regulated TXNIP expression.

Echo Biotech Role

Echo Biotech contributed targeting-peptide/surface modification, EV material/reference-material supply; the study also used or cited ExoBrooch™, ContrExo®.

Related platforms: Echosome®, EV Materials / Reference Materials, Research Reagents & Tools

Related services and capabilities: Targeting Peptide / Surface Modification, EV Material / Reference Material Supply

Related products or reagents: ExoBrooch™ Targeting Peptide/Lipid Anchor Kit, ContrExo® EV Reference Material

References

Original publication: Exosome-based WTAP siRNA delivery ameliorates myocardial ischemia-reperfusion injury Eur J Pharm Biopharm. 2024. DOI: 10.1016/j.ejpb.2024.114218. PMID/PMCID: 38367759.