Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma

Quick Facts

  • Publication title: Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma
  • Journal: BLOOD SCI
  • Year: 2023
  • DOI: 10.1097/BS9.0000000000000160
  • PMID/PMCID: 37546707; PMC10400059

Research overview

Our study aimed at identifying the expression profile of exosomal miRNAs (exo-miRNAs) in the serum of multiple myeloma (MM) patients and investigating the regulation networks and their potential functions by integrated bioinformatics analysis. 13 core exo-miRNA target genes were applied to create a novel prognostic signature to provide risk stratification for MM patients, which is associated with the immune microenvironment of MM patients.

Key findings

MicroRNAs (MiRNAs) carried by exosomes play pivotal roles in the crosstalk between cell components in the tumor microenvironment.

Our study aimed at identifying the expression profile of exosomal miRNAs (exo-miRNAs) in the serum of multiple myeloma (MM) patients and investigating the regulation networks and their potential functions by integrated bioinformatics analysis.

Exosomes in serum from 19 newly diagnosed MM patients and 9 healthy donors were isolated and the miRNA profile was investigated by small RNA sequencing.

Differential expression of exo-miRNAs was calculated and target genes of miRNAs were predicted.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma Blood science. 2023. DOI: 10.1097/BS9.0000000000000160. PMID/PMCID: 37546707; PMC10400059.