Exosome-transmitted microRNA-323a-3p participated in the occurrence of Hirschsprung's disease
Quick Facts
- Publication title: Exosome-transmitted microRNA-323a-3p participated in the occurrence of Hirschsprung's disease
- Journal: Epigenomics
- Year: 2026
- DOI: 10.1080/17501911.2026.2647714
- PMID/PMCID: 41906498; PMC13097784
Research overview
We assessed the effects of exosomal miR-323a-3p on the proliferation and migration of immortalized ENCC-derived neural cell line (iENC) in vitro using CCK-8, EdU and Transwell assays, and its impact on ENCC migration in vivo using a zebrafish model. Our results suggest that measuring plasma exosomal miR-323a-3p in blood could help doctors diagnose HSCR earlier and that the miR-323a-3p/TET2 pathway may be a target for future treatments.
Key findings
Exosomal miR-323a-3p was taken up by iENCs and suppressed their proliferation and migration.
TET2 was identified as a potential miR-323a-3p target.
TET2 was downregulated in HSCR aganglionic tissues, and its knockdown inhibited iENC proliferation and migration.
In the zebrafish model, exosomal miR-323a-3p impaired distal ENCC colonization.
Study design
Plasma exosomes and colon tissues were collected from HSCR patients and controls.
We assessed the effects of exosomal miR-323a-3p on the proliferation and migration of immortalized ENCC-derived neural cell line (iENC) in vitro using CCK-8, EdU and Transwell assays, and its impact on ENCC migration in vivo using a zebrafish model.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Exosome-transmitted microRNA-323a-3p participated in the occurrence of Hirschsprung's disease Epigenomics. 2026. DOI: 10.1080/17501911.2026.2647714. PMID/PMCID: 41906498; PMC13097784.