Exploration of patient plasma exosomes as biomarkers for predicting lung cancer brain metastasis
Quick Facts
- Publication title: Exploration of patient plasma exosomes as biomarkers for predicting lung cancer brain metastasis
- Journal: FRONT MED
- Year: 2025
- DOI: 10.3389/fmed.2025.1687866
- PMID/PMCID: 41416095; PMC12709389
Research overview
Many studies have found that exosomes have numerous advantages in the early diagnosis of tumors. Plasma exosomal miR-223-3p, miR-27a-3p, miR-27b-3p, and IBA, IVA, VA, and AA in advanced patients are closely associated with brain metastasis and have the potential to act as biomarkers for predicting brain metastasis in lung cancer patients.
Key findings
Exosomal miR-223-3p, miR-27a-3p, and miR-27b-3p were significantly increased in the plasma exosomes of lung cancer patients with brain metastasis.
The concentrations of isobutyric acid (IBA), valeric acid (VA), isovaleric acid (IVA), and acetic acid (AA) were markedly elevated in the plasma exosomes of lung cancer patients with brain metastasis.
Spearman correlation analysis revealed that both miR-27a-3p and miR-27b-3p had significant associations with IVA and VA.
Study design
The total RNA of plasma exosomes of advanced lung cancer patients was extracted and sequenced.
The BLAST software was used to align the predicted target gene sequence against the GO and KEGG databases, thereby acquiring annotation details for the target genes.
The selected exosomal miRNAs and short-chain fatty acids were subjected to diagnostic performance validation analysis.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, EV metabolomics/lipidomics, qPCR validation; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Metabolomics/Lipidomics, qPCR Validation, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Exploration of patient plasma exosomes as biomarkers for predicting lung cancer brain metastasis Frontiers in medicine. 2025. DOI: 10.3389/fmed.2025.1687866. PMID/PMCID: 41416095; PMC12709389.