Exploring the impact of follicular fluid exosomes on porcine cumulus cell and oocyte function: Regulatory network analysis based on the ENSSSCG00000042788/ssc-miR-504/PDHA1 axis
Quick Facts
- Publication title: Exploring the impact of follicular fluid exosomes on porcine cumulus cell and oocyte function: Regulatory network analysis based on the ENSSSCG00000042788/ssc-miR-504/PDHA1 axis
- Journal: Theriogenology
- Year: 2025
- DOI: 10.1016/j.theriogenology.2025.117556
- PMID/PMCID: 40614304
Research overview
In this study, we conducted transcriptomic analysis on cumulus cells cultured with varying exosome concentrations and identified 334 differentially expressed long noncoding RNAs (lncRNAs). Functional enrichment analysis indicated that the differentially expressed lncRNAs may be involved in cumulus cell expansion, oocyte maturation, and embryo development.
Key findings
The development and maturation of mammalian oocytes are crucial stages in reproduction, directly influencing embryo health and the genetic quality of offspring.
Follicular fluid serves as the microenvironment for oocyte development, with exosomes playing a key role in regulating the activities of cumulus cells and oocytes.
However, the mechanisms governing porcine oocyte maturation remain incompletely understood.
In this study, we conducted transcriptomic analysis on cumulus cells cultured with varying exosome concentrations and identified 334 differentially expressed long noncoding RNAs (lncRNAs).
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, whole-transcriptome sequencing.
Related platforms: Exoomics®
Related services and capabilities: Biofluid EV Isolation & Purification, EV Whole-Transcriptome Sequencing
References
Original publication: Exploring the impact of follicular fluid exosomes on porcine cumulus cell and oocyte function: Regulatory network analysis based on the ENSSSCG00000042788/ssc-miR-504/PDHA1 axis Theriogenology. 2025. DOI: 10.1016/j.theriogenology.2025.117556. PMID/PMCID: 40614304.