Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma

Quick Facts

  • Publication title: Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma
  • Journal: J Transl Med
  • Year: 2021
  • DOI: 10.1186/s12967-021-02775-9
  • PMID/PMCID: 33712033; PMC7953782

Research overview

Extracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in liquid biopsy. Due to the complex biological composition of plasma, plasma RNA omics analysis (RNomics) is easily affected, thus it is necessary to select an optimal strategy from exiting methods according to the performance for intended application.

Key findings

The results showed that the lowest miRNA binding protein AGO2 (Argonaute-2) and the highest EVs-specific miRNA and lncRNA were observed in EVs obtained through SEC, meanwhile the content of the non-specific miRNA was the lowest.

Further RNA-Seq data revealed that RNAs obtained via SEC presented more useful reads for both miRNA and mRNA.

Furthermore, the mRNA delivered via SEC tended to have a concentration comparable to the ideal FPKM (Fragments Per Kilobase Million) value.

Study design

ultracentrifugation (UC), size exclusion chromatography (SEC), and two most frequently-used commercially available isolation kit (ExoQuick and exoEasy).

We compared the yield, purity, PCR quantification of RNAs, miRNA-seq analyses and mRNA-seq analyses of RNAs from EVs isolated using four methods.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, whole-transcriptome sequencing; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, EV Whole-Transcriptome Sequencing, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma J Transl Med. 2021. DOI: 10.1186/s12967-021-02775-9. PMID/PMCID: 33712033; PMC7953782.