Extraction and identification of synovial tissue-derived exosomes by different separation techniques
Quick Facts
- Publication title: Extraction and identification of synovial tissue-derived exosomes by different separation techniques
- Journal: J Orthop Surg Res
- Year: 2020
- DOI: 10.1186/s13018-020-01604-x
- PMID/PMCID: 32151262; PMC7063768
Research overview
The aim of this study is to compare the efficiency of different separation techniques for extracting synovial tissue-derived exosomes. The synovial tissue discarded during knee arthroscopy or total knee arthroplasty surgery was collected from the Third Affiliated Hospital of Beijing University of Chinese Medicine.
Key findings
The extracts of enriched round and discoid vesicles were successfully extracted with UC, SECF, and PEG.
The results of TEM have shown that all three extraction methods can extract circular or elliptical vesicles with disc- and cup-shaped structures from the synovial tissue, with the diameter is about 30-150 nm.
NTA suggested the main peaks of three groups of exosomes are around 100-120 nm, and the concentration of the three groups of exosomes was greater than 1 × 1010/ml.
The results of WB showed that three positive protein markers (CD9, CD63, and Flotillin-1) were highly expressed in the suspension extracted by the three methods and low in the synovial tissue.
Study design
The synovial tissue discarded during knee arthroscopy or total knee arthroplasty surgery was collected from the Third Affiliated Hospital of Beijing University of Chinese Medicine.
Ultracentrifugation (UC), filtration combined with size exclusion chromatography (SECF), and 8% polyethylene glycol (PEG) were used to extract synovial tissue-derived exosomes.
Transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA), and Western Blot (WB) were used to detect the morphology, particle size, and biomarker proteins (CD9, CD63, Flotillin-1, and calnexin) of exosomes.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Extraction and identification of synovial tissue-derived exosomes by different separation techniques J Orthop Surg Res. 2020. DOI: 10.1186/s13018-020-01604-x. PMID/PMCID: 32151262; PMC7063768.