Genomic instability-derived plasma extracellular vesicle-microRNA signature as a minimally invasive predictor of risk and unfavorable prognosis in breast cancer

Quick Facts

  • Publication title: Genomic instability-derived plasma extracellular vesicle-microRNA signature as a minimally invasive predictor of risk and unfavorable prognosis in breast cancer
  • Journal: J Nanobiotechnol
  • Year: 2021
  • DOI: 10.1186/s12951-020-00767-3
  • PMID/PMCID: 33436002; PMC7802300

Research overview

Then, we constructed a discovery and validation study with multicenter prospective cohorts. Recent studies have indicated that microRNA (miRNA) regulation in genomic instability (GI) is associated with disease risk and clinical outcome.

Key findings

We identified a GI-derived three miRNA signature (MIR421, MIR128-1 and MIR128-2) in the serum extracellular vesicles of BC patients, which was significantly associated with poor prognosis in all the cohorts tested and remained as an independent prognostic factor using multivariate analyses.

When integrated with the clinical characteristics, the composite miRNA-clinical prognostic indicator showed improved prognostic performance.

772 in GSE73002, GSE41922 and TCGA cohorts, respectively.

Furthermore, circulating EVs from BC patients in the in-house cohort harbored elevated levels of miGISig, with effective diagnostic accuracy.

Study design

Integrative analysis of miRNA expression and somatic mutation profiles was performed to identify GI-associated miRNAs.

Then, we constructed a discovery and validation study with multicenter prospective cohorts.

The GI-derived miRNA signature (miGISig) was developed in the TCGA discovery cohort (n = 261), and was subsequently independently validated in internal TCGA validation (n = 261) and GSE22220 (n = 210) cohorts for prognosis prediction, and in GSE73002 (n = 3966), GSE41922 (n = 54), and in-house clinical exosome (n = 30) cohorts for diagnostic performance.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification.

Related platforms: Exoomics®

Related services and capabilities: Biofluid EV Isolation & Purification

References

Original publication: Genomic instability-derived plasma extracellular vesicle-microRNA signature as a minimally invasive predictor of risk and unfavorable prognosis in breast cancer J Nanobiotechnol. 2021. DOI: 10.1186/s12951-020-00767-3. PMID/PMCID: 33436002; PMC7802300.