Gp130 Promotes Inflammation via the STAT3/JAK2 Pathway in Allergic Conjunctivitis
Quick Facts
- Publication title: Gp130 Promotes Inflammation via the STAT3/JAK2 Pathway in Allergic Conjunctivitis
- Journal: INVEST OPHTH VIS SCI
- Year: 2023
- DOI: 10.1167/iovs.64.4.5
- PMID/PMCID: 37022703; PMC10082384
Research overview
We found that gp130 is essential for AC, as gp130 levels are elevated in AC. Inhibiting gp130 phosphorylation alleviates ocular surface inflammation in mice, presenting a potential treatment approach for AC.
Key findings
Gp130 is upregulated in the conjunctival tissues of OVA-induced mice, the serum and tears of patients, and the histamine-stimulated HConEpiCs.
Signal transducer and activator of transcription 3 (STAT3) and Janus kinase 2 (JAK2) were upregulated in the conjunctival tissues of mice with OVA-induced AC and in HConEpiCs.
Ocular surface inflammation was significantly relieved in LMT-28-treated mice.
The expression of IgE, IL-4, IL-5, and IL-13 in serum of LMT-28-treated mice decreased.
Study design
To compare mRNA expression profiles, the conjunctival tissues of BALB/c mice with ovalbumin (OVA)-induced AC were subjected to RNA-sequencing (RNA-seq) analysis followed by bioinformatic analysis.
A nonrandomized study involving 57 patients with AC and 24 sex- and age-matched healthy individuals was conducted.
A protein chip was used to detect cytokine levels in patient tears.
Echo Biotech Role
Echo Biotech contributed whole-transcriptome sequencing.
Related platforms: Exoomics®
Related services and capabilities: EV Whole-Transcriptome Sequencing
References
Original publication: Gp130 Promotes Inflammation via the STAT3/JAK2 Pathway in Allergic Conjunctivitis Investigative ophthalmology & visual science. 2023. DOI: 10.1167/iovs.64.4.5. PMID/PMCID: 37022703; PMC10082384.