Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis
Quick Facts
- Publication title: Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis
- Journal: J Orthop Surg Res
- Year: 2023
- DOI: 10.1186/s13018-023-04020-z
- PMID/PMCID: 37496029; PMC10373377
Research overview
In this study, we aim to identify PMOP-associated circulating exosomal miRNAs and evaluate their diagnostic performance. These results suggested that the plasma exosomal miRNAs had the potential to serve as noninvasive diagnostic biomarkers for PMOP.
Key findings
Twenty-seven miRNAs were identified as differentially expressed in PMOP versus controls in sequencing data, of which six exosomal miRNAs (miR-196-5p, miR-224-5p, miR320d, miR-34a-5p, miR-9-5p, and miR-98-5p) were confirmed to be differentially expressed in PMOP patients by qRT-PCR in the validation cohort.
In addition, the number of pregnancies was found to be an independent risk factor that can improve the performance of exosomal miRNAs in diagnosing PMOP.
Study design
We performed next-generation sequencing and bioinformatics analysis of plasma exosomal miRNAs from 12 PMOP patients and 12 non-osteoporosis controls to identify PMOP-associated exosomal miRNAs, and then validated them in an independent natural community cohort with 26 PMOP patients and 21 non-osteoporosis controls.
Exosomes were isolated with the size exclusion chromatography method from the plasma of elder postmenopausal women.
The plasma exosomal miRNA profiles were characterized in PMOP paired with controls with next-generation sequencing.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, qPCR validation; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Identification and evaluation of circulating exosomal miRNAs for the diagnosis of postmenopausal osteoporosis J Orthop Surg Res. 2023. DOI: 10.1186/s13018-023-04020-z. PMID/PMCID: 37496029; PMC10373377.