Identification of exosomal miRNAs associated with the anthracycline-induced liver injury in postoperative breast cancer patients by small RNA sequencing

Quick Facts

  • Publication title: Identification of exosomal miRNAs associated with the anthracycline-induced liver injury in postoperative breast cancer patients by small RNA sequencing
  • Journal: PeerJ
  • Year: 2020
  • DOI: 10.7717/peerj.9021
  • PMID/PMCID: 32355577; PMC7185038

Research overview

Seven post-chemotherapy patients were recruited in this study. Moreover, our study provided an experimental basis for experimental verification to reveal the actual function and mechanism of miRNAs in AILI.

Key findings

A total of 30 DE-miRNAs and 79 AILI-related target genes were identified.

AILI-related target genes of the DE-miRNAs are significantly enriched in NOD-like receptor signaling pathway, the HIF-1 signaling pathway, and the FoxO signaling pathway.

Then, the hub genes were screened and we discovered that IL-6 and SOD2 are the most critical genes that may be involved in the development of AILI through the activation of immune response and the occurrence of oxidative stress, respectively.

In addition, we found that miR-1-3p could potentially regulate most of the hub genes in the miRNA-hub gene network.

Study design

Seven post-chemotherapy patients were recruited in this study.

After isolated plasma-derived exosomes, small RNA sequencing revealed exosomal miRNA profiles and differentially expressed miRNAs (DE-miRNAs) were identified between the liver injury group and non-liver injury group.

miRTarBase and miRDB were used to predict the potential target genes of DE-miRNAs.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis.

Related platforms: Exoomics®

Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing

References

Original publication: Identification of exosomal miRNAs associated with the anthracycline-induced liver injury in postoperative breast cancer patients by small RNA sequencing PeerJ. 2020. DOI: 10.7717/peerj.9021. PMID/PMCID: 32355577; PMC7185038.