Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma
Quick Facts
- Publication title: Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma
- Journal: EBioMedicine
- Year: 2022
- DOI: 10.1016/j.ebiom.2022.104281
- PMID/PMCID: 36162205; PMC9519476
Research overview
This study revealed the spatial landscape of various cell subsets within the TME and the potential cross-talk among diverse cells, which provides novel insights into cancer intervention. Although distinct cell subsets can be identified via single-cell RNA sequencing (scRNA-seq), the spatial composition of cells within the TME is difficult to characterise.
Key findings
The subpopulations of seven stromal cells were identified within the TME of ESCC, and the architecture of scRNA-seq-determined subsets was mapped in cancer and stromal regions.
The distribution of various stromal cells and their subpopulations was heterogeneous.
Compared with immune cells, non-immune stromal cells were significantly enriched in the TME.
Most subsets of epithelial cells were enriched in the cancer regions, whereas inflammatory cancer-associated fibroblasts were correlated with the stromal regions.
Study design
Tissue samples were collected from three patients with esophageal squamous cell carcinoma (ESCC), and scRNA-seq was performed to identify distinct cell subsets.
In addition, a microarray-based spatial transcriptomics (ST) method was used to characterise the spatial landscape of expression data via an array of spots.
Using multimodal intersection analysis (MIA) to integrate scRNA-seq and ST, the exact cellular components of the tumor and stromal regions were annotated.
Echo Biotech Role
Echo Biotech contributed single-cell RNA sequencing, spatial transcriptomics.
Related platforms: Exoomics®
Related services and capabilities: Single-Cell RNA Sequencing, Spatial Transcriptomics
References
Original publication: Integrating microarray-based spatial transcriptomics and single-cell RNA-sequencing reveals tissue architecture in esophageal squamous cell carcinoma EBioMedicine. 2022. DOI: 10.1016/j.ebiom.2022.104281. PMID/PMCID: 36162205; PMC9519476.