Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies
Quick Facts
- Publication title: Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies
- Journal: Adv Healthc Mater
- Year: 2023
- DOI: 10.1002/adhm.202303430
- PMID/PMCID: 37942845
Research overview
The isolation and enrichment of specific extracellular vesicle (EV) subpopulations are essential in the context of precision medicine. However, the current methods predominantly rely on a single-positive marker and are susceptible to interference from soluble proteins or impurities.
Key findings
The isolation and enrichment of specific extracellular vesicle (EV) subpopulations are essential in the context of precision medicine.
However, the current methods predominantly rely on a single-positive marker and are susceptible to interference from soluble proteins or impurities.
This limitation represents a significant obstacle to the widespread application of EVs in biological research.
Herein, a novel approach that utilizes proximity ligation assay (PLA) and DNA-RNA hybridization are proposed to facilitate the binding of two proteins on the EV membrane in advance enabling the isolation and enrichment of intact EVs with double-positive membrane proteins followed by using functionalized magnetic beads for capture and enzymatic cleavage for isolated EVs release.
Echo Biotech Role
Echo Biotech contributed miRNA profiling/sequencing and bioinformatic analysis.
Related platforms: Exoomics®
Related services and capabilities: EV miRNA Profiling / Sequencing
References
Original publication: Isolation and enrichment of extracellular vesicles with double-positive membrane protein for subsequent biological studies Adv Healthc Mater. 2023. DOI: 10.1002/adhm.202303430. PMID/PMCID: 37942845.