A Label-Free Liquid Chromatography–Tandem Mass Spectrometry Method for the Quantitative Analysis of Exosome Pharmacokinetics In Vivo
Quick Facts
- Publication title: A Label-Free Liquid Chromatography–Tandem Mass Spectrometry Method for the Quantitative Analysis of Exosome Pharmacokinetics In Vivo
- Journal: Pharmaceutics
- Year: 2025
- DOI: 10.3390/pharmaceutics17060699
- PMID/PMCID: 40574012; PMC12195665
Research overview
Methods: To address this challenge, we developed a label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to investigate the PK of naive exosome-based therapeutic modalities. Due to their complex and heterogeneous composition, most existing PK methods rely on chemical or genetic labeling, which may alter their native behavior and complicate accurate analysis.
Key findings
Background: Exosomes are nanoscale extracellular vesicles actively secreted by cells that play critical roles in disease biomarker discovery, drug delivery, and direct therapeutic applications.
However, in vivo pharmacokinetic (PK) studies of exosomes remain limited, hindering their clinical translation.
Due to their complex and heterogeneous composition, most existing PK methods rely on chemical or genetic labeling, which may alter their native behavior and complicate accurate analysis.
Methods: To address this challenge, we developed a label-free liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to investigate the PK of naive exosome-based therapeutic modalities.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV material/reference-material supply, EV pharmacokinetic analysis support; the study also used or cited Exosupur®, ContrExo®.
Related platforms: Exoomics®, EchoPharm®, EV Materials / Reference Materials, Research Reagents & Tools
Related services and capabilities: Biofluid EV Isolation & Purification, EV Material / Reference Material Supply, EV Pharmacokinetics Support
Related products or reagents: Exosupur® EV Isolation/Purification Kit, ContrExo® EV Reference Material
References
Original publication: A Label-Free Liquid Chromatography–Tandem Mass Spectrometry Method for the Quantitative Analysis of Exosome Pharmacokinetics In Vivo Pharmaceutics. 2025. DOI: 10.3390/pharmaceutics17060699. PMID/PMCID: 40574012; PMC12195665.