LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis
Quick Facts
- Publication title: LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis
- Journal: Onco Targets Ther
- Year: 2020
- DOI: 10.2147/OTT.S282053
- PMID/PMCID: 33262606; PMC7700090
Research overview
The objective of this study is to reveal the dysregulated lncRNAs in OS plasma exosomes and explore their functions in OS. Further assays indicated that CASC15 knockdown could restrain the proliferation, migration, and invasion of OS cells, and inhibit the growth of OS in xenograft models.
Key findings
Upregulated CASC15 was observed in OS plasma exosomes compared with control, and the same expression was observed in the OS tissues and cell lines.
Further assays indicated that CASC15 knockdown could restrain the proliferation, migration, and invasion of OS cells, and inhibit the growth of OS in xenograft models.
Furthermore, our results demonstrated CASC15 regulated OS progression via acting as miR-338-3p sponge, and RAB14 was a direct downstream target of miR-338-3p.
Rescue experiments verified CASC15 promotes OS cell growth and metastasis by upregulating RAB14 expression.
Study design
Microarray was performed to analyze dysregulated exosomal lncRNAs.
Western blot, qRT-PCR assays, and Dual-luciferase reporter assay were used to verify the interaction among cancer susceptibility 15 (CASC15), miR-338-3p, and RAB14.
Cck-8, colony formation assay, and transwell assay were performed to explore and characterize the effects of CASC15 on OS cells.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: LncRNA CASC15 is Upregulated in Osteosarcoma Plasma Exosomes and CASC15 Knockdown Inhibits Osteosarcoma Progression by Regulating miR-338-3p/RAB14 Axis Onco Targets Ther. 2020. DOI: 10.2147/OTT.S282053. PMID/PMCID: 33262606; PMC7700090.