Lung Tissue Extracellular Vesicles-Mediated Delivery of miR-128-3p as a Novel Mechanism of Acute Lung Inflammation

Quick Facts

  • Publication title: Lung Tissue Extracellular Vesicles-Mediated Delivery of miR-128-3p as a Novel Mechanism of Acute Lung Inflammation
  • Journal: Int J Nanomedicine
  • Year: 2025
  • DOI: 10.2147/IJN.S510241
  • PMID/PMCID: 40255671; PMC12009125

Research overview

This study combines transcriptomic profiling and functional validation to reveal how Lung Ti-EVs mediate macrophage polarization through miRNA-dependent NLRP3 inflammasome activation. This study combines transcriptomic profiling and functional validation to reveal how Lung Ti-EVs mediate macrophage polarization through miRNA-dependent NLRP3 inflammasome activation.

Key findings

We identified extracellular vesicles (EVs) enriched in lung tissues from septic ALI mice, selectively carrying miRNAs including miR-128-3p.

In vivo administration of these EVs exacerbated pulmonary inflammation by expanding M1 macrophage populations, while in vitro experiments demonstrated EV-mediated miR-128-3p delivery to macrophages stimulated TNF-α and IL-6 production.

Mechanistically, miR-128-3p promoted macrophage proliferation and inflammatory responses by targeting Rab20.

Study design

We established a sepsis mouse model, extracted and characterized lung tissue-derived EVs, performed high-throughput transcriptome sequencing and bioinformatics analysis.

Intratracheal administration of these EVs to wild-type C57BL/6 mice revealed their effects on pulmonary inflammation, macrophage polarization, and proliferation.

7 macrophages further validated these findings and explored underlying mechanisms.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Lung Tissue Extracellular Vesicles-Mediated Delivery of miR-128-3p as a Novel Mechanism of Acute Lung Inflammation International journal of nanomedicine. 2025. DOI: 10.2147/IJN.S510241. PMID/PMCID: 40255671; PMC12009125.