miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients
Quick Facts
- Publication title: miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients
- Journal: Cell Death Discov
- Year: 2023
- DOI: 10.1038/s41420-023-01328-x
- PMID/PMCID: 36681676; PMC9867758
Research overview
The present study focused on the in-depth miRNA expression profiles of neutrophil-derived extracellular vesicles and explored the potential molecular biomarkers during the process of SIC. miR-150-5p may be a predictor of sepsis severity development but warrants further study.
Key findings
Sixty-eight miRNAs from neutrophil-derived extracellular vesicles were changed significantly between healthy controls and without septic cardiomyopathy patients (61 miRNAs upregulated and seven downregulated).
Thirty-eight miRNAs were differentially expressed in the septic cardiomyopathy patients.
27 common differentially expressed miRNAs were found in both groups with similar kinetics (23 miRNAs upregulated and four downregulated).
The enriched cellular signaling pathway mediated by miRNAs from sepsis to septic cardiomyopathy was the HIF-1 signaling system modulated septic inflammation.
Study design
Neutrophil-derived extracellular vesicles were isolated from the blood samples in three sepsis patients with or without cardiomyopathy on day 1 and day 3 after ICU admission in comparison with three healthy controls.
miRNAs were determined by RNA sequencing.
The closely related differentially expressed miRNAs with SIC were further validated through qRT-PCR in the other cohorts of sepsis patients with (30 patients) or without cardiomyopathy (20 patients) and the association between miRNAs and the occurrence or disease severity of septic cardiomyopathy were stratified with logistic regression analysis.
Echo Biotech Role
Echo Biotech contributed EV characterization, miRNA profiling/sequencing and bioinformatic analysis.
Related platforms: Exoomics®
Related services and capabilities: EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing
References
Original publication: miR-150-5p in neutrophil-derived extracellular vesicles associated with sepsis-induced cardiomyopathy in septic patients Cell Death Discov. 2023. DOI: 10.1038/s41420-023-01328-x. PMID/PMCID: 36681676; PMC9867758.