MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma

Quick Facts

  • Publication title: MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma
  • Journal: Front Immunol
  • Year: 2025
  • DOI: 10.3389/fimmu.2025.1619448
  • PMID/PMCID: 40948741; PMC12427126

Research overview

This study demonstrates that platelet miRNAs are a superior source for liquid biopsy in LAC compared to exosomal miRNAs. Critically, the well-documented interaction between tumor-derived exosomes and platelets, which complicates exosome isolation due to inevitable platelet contamination, raises a pivotal question: Could the analysis of platelet miRNA, a far easier-to-isolate component, offer a viable and efficient alternative to exosome-based diagnostics?

Key findings

Platelets contained significantly greater miRNA diversity and abundance compared to exosomes.

Differentially expressed platelet miRNAs showed higher concordance with tissue-specific signatures than their exosomal counterparts.

hsa-let-7i-5p was identified as the most stable reference miRNA for normalizing platelet miRNA expression in LAC.

hsa-miR-199b-3p was significantly downregulated in the platelets of LAC patients compared to both HD and, crucially, BPN patients.

Study design

We performed miRNA sequencing (miRNA-seq) on paired peripheral blood platelets and plasma exosomes from healthy donors (HD), BPN, and LAC patients.

The abundance and diversity of miRNAs were compared.

Candidate reference miRNAs for platelet studies were screened and validated using RT-qPCR and multiple stability algorithms.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis.

Related platforms: Exoomics®

Related services and capabilities: Biofluid EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing

References

Original publication: MiRNA sequencing of platelet and exosome revealed platelet miR-199b-3p as a potential biomarker in lung adenocarcinoma Frontiers in immunology. 2025. DOI: 10.3389/fimmu.2025.1619448. PMID/PMCID: 40948741; PMC12427126.