Mitochondria-derived vesicles serve as reactors in response to stress in cardiomyocytes
Quick Facts
- Publication title: Mitochondria-derived vesicles serve as reactors in response to stress in cardiomyocytes
- Journal: Cell Commun Signal
- Year: 2025
- DOI: 10.1186/s12964-025-02547-8
- PMID/PMCID: 41413833; PMC12825251
Research overview
Nanoparticle tracking analysis (NTA) was used to assess the size and quantity of MDVs. Strictly-organized adult cardiomyocytes contain abundant mitochondria; however, the precise characteristics and functions of MDVs in the heart remain unclear.
Key findings
MDVs were observed in live rat cardiomyocytes with TEM revealing vesicles 70-150 nm in diameter.
MDVs were universally distributed, showed active motility, and colocalized with other intracellular organelles in adult cardiomyocytes.
Proteomics analysis showed MDV-associated proteins and found that the respiratory functions of MDVs were preserved in cardiomyocytes.
The number of MDVs in cardiomyocytes was found to increase in response to various acute physiological and pathological stimuli.
Study design
Rat cardiomyocytes were examined using transmission electron microscopy (TEM) to visualize MDVs.
Live-cell imaging with STED microscopy and fluorescent mitochondrial dyes (PK Mito Red and PK Mito Deep Red) was performed to track MDV dynamics in neonatal and adult rat cardiomyocytes.
Nanoparticle tracking analysis (NTA) was used to assess the size and quantity of MDVs.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV proteomics.
Related platforms: Exoomics®
Related services and capabilities: EV Isolation & Purification, EV Proteomics
References
Original publication: Mitochondria-derived vesicles serve as reactors in response to stress in cardiomyocytes Cell communication and signaling. 2025. DOI: 10.1186/s12964-025-02547-8. PMID/PMCID: 41413833; PMC12825251.