MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression
Quick Facts
- Publication title: MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression
- Journal: J Transl Med
- Year: 2025
- DOI: 10.1186/s12967-025-06776-w
- PMID/PMCID: 40817243; PMC12355852
Research overview
M2 macrophages are known to enhance the malignant phenotype of breast cancer (BC) cells, yet the underlying mechanisms remain unclear. Targeting this feedback loop offers a potential therapeutic strategy for BC.
Key findings
Maestro (MRO) was involved in the crosstalk between M2 macrophages and BC cells.
MRO expression was elevated in BC tissues, particularly in those with high CD68 and CD206 expression, and correlated with poor prognosis.
MRO was highly expressed in M2 macrophages, and its mRNA was transferred from M2 macrophages to BC cells via exosomes.
Engineering exosomes with MRO knockdown demonstrated reduced tumor-promoting effects compared to controls.
Study design
scRNA sequencing analysis, chromatin immunoprecipitation, immunoprecipitation, exosome isolation, and biological experiments were used to analyze this crosstalk.
Echo Biotech Role
Echo Biotech contributed cargo loading; the study also used or cited ExoLoad®.
Related platforms: Echosome®, Research Reagents & Tools
Related services and capabilities: Small RNA / Cargo Loading, Research Reagent / Product Supply
Related products or reagents: ExoLoad® Nucleic Acid Loading Kit
References
Original publication: MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression Journal of translational medicine. 2025. DOI: 10.1186/s12967-025-06776-w. PMID/PMCID: 40817243; PMC12355852.