Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC
Quick Facts
- Publication title: Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC
- Journal: Stem Cell Res Ther
- Year: 2023
- DOI: 10.1186/s13287-023-03491-5
- PMID/PMCID: 37749641; PMC10521478
Research overview
Our study first focused on the transcriptomic profile of hUMSC-EVs based on nanopore sequencing. Up to date, the knowledge of their RNA components is limited to NGS sequencing and cannot provide a comprehensive transcriptomic landscape, especially the long and full-length transcripts.
Key findings
34% of which transcripts were fragmented.
A series of full-length and long transcripts was also observed and showed a significantly higher proportion of intact or near-complete transcripts in exosomes than that in microvesicles derived from hUMSCs.
Although the composition of RNA biotypes transported by different EV subtypes was similar, the distribution of transcripts and genes revealed the inter-heterogeneity and intra-stability between exosomes and microvesicles.
Further, 85 different expressed transcripts (56 genes) and 7 fusion genes were identified.
Study design
In this study, different EV subtypes (exosomes and microvesicles) derived from hUMSCs were isolated and identified by density gradient centrifugation.
Subsequently, the realistic long transcriptomic profile in different subtypes of hUMSC-EVs was systematically compared by nanopore sequencing and bioinformatic analysis.
Echo Biotech Role
Echo Biotech contributed EV characterization.
Related platforms: Exoomics®
Related services and capabilities: EV Basic Characterization (TEM/NTA/WB)
References
Original publication: Nano-seq analysis reveals different functional tendency between exosomes and microvesicles derived from hUMSC Stem Cell Res Ther. 2023. DOI: 10.1186/s13287-023-03491-5. PMID/PMCID: 37749641; PMC10521478.