A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer

Quick Facts

  • Publication title: A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer
  • Journal: Cancer Biol Ther
  • Year: 2025
  • DOI: 10.1080/15384047.2025.2593744
  • PMID/PMCID: 41340217; PMC12688251

Research overview

Prostate cancer (PCa) is a major health concern, and current PSA screening is limited by low specificity and the risk of overdiagnosis. Extracellular vesicle (EV)-derived RNA biomarkers offer a promising non-invasive alternative for early detection.

Key findings

TCLN reliably detected the candidate RNA biomarkers from PCa cell lines and clinical samples, with strong concordance to qRT-PCR.

Serum EV RNAs (miR-141, miR-375, Let-7c) and urine EV RNAs (miR-141, miR-375, PCA3 lncRNA, T1-E2) effectively distinguished PCa patients from controls.

741, respectively, surpassing those of prostate-specific antigen (PSA) alone.

Serum miR-141, miR-375, and urine miR-141, miR-375, and PCA3 lncRNA, also showed remarkable correlations with PCa Gleason score (GS), tumor stage, and metastatic status.

Study design

We utilized a tethered cationic lipoplex nanoparticle (TCLN) biochip for amplification-free EV RNA detection at the single-vesicle level.

Eight candidate RNAs (four miRNAs, three mRNAs, and one lncRNA) were profiled in serum and urine samples from PCa patients, benign prostatic hyperplasia (BPH) patients, and healthy controls (HC).

Diagnostic and risk stratification performance was evaluated in discovery and validation cohorts, with qRT-PCR used for validation.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: A novel biochip-based liquid biopsy for extracellular vesicle RNA detection in prostate cancer Cancer biology & therapy. 2025. DOI: 10.1080/15384047.2025.2593744. PMID/PMCID: 41340217; PMC12688251.