Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction
Quick Facts
- Publication title: Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction
- Journal: Brain
- Year: 2026
- DOI: 10.1093/brain/awag049
- PMID/PMCID: 41666156
Research overview
Small extracellular vesicles (sEVs) efficiently transport pathological RNAs in ischemic stroke, yet whether penumbral hypoxia-induced EVs deliver pathogenic cargo to remote regions, impacting acute ischemic stroke outcomes, remains unclear. Using a focal ischemia mouse model, we isolated brain-derived EVs (BDEVs) from the cortical penumbra and determined their pathological impact on synaptically connected remote regions.
Key findings
Small extracellular vesicles (sEVs) efficiently transport pathological RNAs in ischemic stroke, yet whether penumbral hypoxia-induced EVs deliver pathogenic cargo to remote regions, impacting acute ischemic stroke outcomes, remains unclear.
Using a focal ischemia mouse model, we isolated brain-derived EVs (BDEVs) from the cortical penumbra and determined their pathological impact on synaptically connected remote regions.
In vitro, penumbra BDEVs (PEVs) exacerbated recipient neuronal damage, intensifying apoptosis and dendritic injury during oxygen-glucose deprivation/reoxygenation.
In vivo tracing confirmed PEV transport via anatomical projections to remote thalamic neurons, where internalization triggered synaptic loss and apoptosis in distal thalamic nuclei.
Echo Biotech Role
Echo Biotech contributed whole-transcriptome sequencing.
Related platforms: Exoomics®
Related services and capabilities: EV Whole-Transcriptome Sequencing
References
Original publication: Penumbra-derived small extracellular vesicles carry pathogenic RNA driving remote damage after cortical infarction Brain. 2026. DOI: 10.1093/brain/awag049. PMID/PMCID: 41666156.