Plasma-derived exosomal miRNA profiles associated with type 1 diabetes
Quick Facts
- Publication title: Plasma-derived exosomal miRNA profiles associated with type 1 diabetes
- Journal: DIABETES-METAB RES
- Year: 2024
- DOI: 10.1002/dmrr.3774
- PMID/PMCID: 38340050
Research overview
To assess the biomarker potential of exosomal miRNAs for T1D, we measured the expression profiles of plasma-derived exosomal miRNAs in T1D and explored their potential functions by bioinformatic analysis. Our results indicated that plasma-derived exosomal miRNAs could serve as promising diagnostic biomarkers of T1D.
Key findings
In total, 43 differentially expressed miRNAs, among which 34 were upregulated and 9 were downregulated, were identified in T1D.
After correcting for multiple testing using false discovery rate, 11 identified exosomal miRNAs still showed statistical significance.
Among the 5 selected miRNAs, 3 miRNAs (miR-103a-3p, miR-144-5p and miR-454-3p) were successfully validated by qRT-PCR.
The biological analysis-enriched terms included protein autophosphorylation and the Hedgehog signalling pathway.
Study design
47) healthy control subjects.
Exosomal miRNA expression profiles were measured using the Illumina NovaSeq 6000 platform.
With verification by quantitative real-time PCR (qRT-PCR), we used multiple bioinformatics approaches to explore the potential biological functions of the identified differentially expressed miRNAs.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, miRNA profiling/sequencing and bioinformatic analysis, qPCR validation.
Related platforms: Exoomics®
Related services and capabilities: Biofluid EV Isolation & Purification, EV miRNA Profiling / Sequencing, qPCR Validation
References
Original publication: Plasma-derived exosomal miRNA profiles associated with type 1 diabetes DIABETES-METABOLISM RESEARCH AND REVIEWS. 2024. DOI: 10.1002/dmrr.3774. PMID/PMCID: 38340050.