Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus
Quick Facts
- Publication title: Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus
- Journal: Front Immunol
- Year: 2022
- DOI: 10.3389/fimmu.2022.995610
- PMID/PMCID: 36177022; PMC9513134
Research overview
Here, we aimed to identify the plasma-derived exosomal mRNA expression profiles in T1DM and to explore their potential biological functions in T1DM. The identified DEMs might be associated with the pathogenesis of T1DM, and some DEMs have the potential to serve as biomarkers and therapeutic targets for T1DM.
Key findings
In total, 112 DEMs were identified in T1DM, among which 66 mRNAs were upregulated and 46 mRNAs were downregulated.
Four of six candidate exosomal mRNAs were successfully validated by qRT-PCR.
Bioinformatics analysis indicated that these mRNAs were most significantly involved in positive regulation by host viral transcription (GO enrichment analysis) and oxidative phosphorylation (KEGG pathway analysis).
Study design
Plasma-derived exosomes were isolated from 10 patients with T1DM and 10 age- and sex-matched control subjects by size exclusion chromatography methods.
Transmission electron microscopy, nanoparticle tracking analysis, and western blot analysis confirmed the presence of exosomes.
The exosomal mRNAs were analyzed using the Illumina HiSeq platform.
Echo Biotech Role
Echo Biotech contributed lncRNA/mRNA sequencing, qPCR validation.
Related platforms: Exoomics®
Related services and capabilities: EV lncRNA/mRNA Sequencing, qPCR Validation
References
Original publication: Plasma-derived exosomal mRNA profiles associated with type 1 diabetes mellitus Front Immunol. 2022. DOI: 10.3389/fimmu.2022.995610. PMID/PMCID: 36177022; PMC9513134.