PlexinA (PLXNA) as a novel scaffold protein for the engineering of extracellular vesicles

Quick Facts

  • Publication title: PlexinA (PLXNA) as a novel scaffold protein for the engineering of extracellular vesicles
  • Journal: J Extracell Vesicles
  • Year: 2024
  • DOI: 10.1002/jev2.70012
  • PMID/PMCID: 39508411; PMC11541859

Research overview

Extracellular vesicles (EVs) had been described as a next-generation drug delivery system, due to the compelling evidence that they can facilitate the transfer of a variety of biomolecules between cells. The identified scaffold protein PLXNA1 showed great potential in developing therapeutic EVs.

Key findings

Extracellular vesicles (EVs) had been described as a next-generation drug delivery system, due to the compelling evidence that they can facilitate the transfer of a variety of biomolecules between cells.

The most frequently used strategy for loading protein cargoes is the endogenous engineering of EVs through genetic fusion of the protein of interest (POI) and scaffold proteins with high EV-sorting ability.

However, the lack of scaffold proteins had become a major issue hindering the promotion of this technology.

Herein, we proposed novel screening criteria that relax the inclusion requirement of candidate scaffold proteins and eventually identified a new scaffold protein, PLXNA1.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, cargo loading, co-authored scientific research; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Echosome®, Research Reagents & Tools

Related services and capabilities: EV Isolation & Purification, Small RNA / Cargo Loading, Co-authored Scientific Research, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: PlexinA (PLXNA) as a novel scaffold protein for the engineering of extracellular vesicles Journal of Extracellular Vesicles. 2024. DOI: 10.1002/jev2.70012. PMID/PMCID: 39508411; PMC11541859.