Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women

Quick Facts

  • Publication title: Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women
  • Journal: Int J Mol Sci
  • Year: 2022
  • DOI: 10.3390/ijms232012343
  • PMID/PMCID: 36293200; PMC9603981

Research overview

Myometrial contraction is essential for successful delivery. Two proteins, GJA1 and SLC39A14, exist in female blood exosomes and are highly expressed in HMSMCs exosomes, are also upregulated in the laboring myometrium, which verified increased in laboring blood samples, might be novel potential biomarkers for myometrial activation.

Key findings

Myometrial contraction is essential for successful delivery.

Recent studies have highlighted the vital roles of tissue-derived exosomes in disease diagnostic, prognostic, and therapeutic applications; however, the characteristics of uterine myometrium-derived exosomes are unclear.

Here, we successfully isolated exosomes from myometrial tissues, human myometrial smooth muscle cells (HMSMCs), and human umbilical vein endothelial cells (HUVECs), then performed quantitative liquid chromatography-tandem mass spectrometry and miRNA sequencing to investigate the cargo of the exosomes.

Fifty-two proteins and five miRNAs were differentially expressed (DE) in term non-labor and term labor myometrium-derived exosomes.

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, EV proteomics; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, EV Proteomics, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Proteomic and miRNA Profiles of Exosomes Derived from Myometrial Tissue in Laboring Women Int J Mol Sci. 2022. DOI: 10.3390/ijms232012343. PMID/PMCID: 36293200; PMC9603981.