Proteomics-based identification of proteins in tumor-derived exosomes as candidate biomarkers for colorectal cancer
Quick Facts
- Publication title: Proteomics-based identification of proteins in tumor-derived exosomes as candidate biomarkers for colorectal cancer
- Journal: WORLD J GASTRO ONCOL
- Year: 2023
- DOI: 10.4251/wjgo.v15.i7.1227
- PMID/PMCID: 37546562; PMC10401461
Research overview
There is an urgent need to find reliable diagnostic biomarkers of CRC and explore the underlying molecular mechanisms. Six exosomal proteins, NHP2, OLFM4, TOP1, SAMP, TAGL and TRIM28, may be promising diagnostic markers and effective therapeutic targets for CRC, but further experimental investigation is needed.
Key findings
A total of 1393 proteins were identified in the CRC tissue group, 1304 proteins were identified in the adjacent tissue group, and 283 proteins were significantly differentially expressed between them.
Enrichment analysis revealed that DEPs were involved in multiple biological processes related to cytoskeleton construction, cell movement and migration, immune response, tumor growth and telomere metabolism, as well as ECM-receptor interaction, focal adhesion and mTOR signaling pathways.
Six differentially expressed exosomal proteins (NHP2, OLFM4, TOP1, SAMP, TAGL and TRIM28) were validated by PRM analysis and evaluated by receiver operating characteristic curve (ROC) analysis.
05) for NHP2, OLFM4, TOP1, SAMP, TAGL, and TRIM28, respectively, indicating their good ability to distinguish CRC tissues from adjacent intestinal tissues.
Study design
In this study, 10 patients over 50 years old who were diagnosed with moderately differentiated adenocarcinoma were recruited.
We paired CRC tissues and adjacent normal intestinal tissues (> 5 cm) to form the experimental and control groups.
Purified exosomes were extracted separately from each tissue sample.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, EV proteomics; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV Proteomics, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Proteomics-based identification of proteins in tumor-derived exosomes as candidate biomarkers for colorectal cancer World journal of gastrointestinal oncology. 2023. DOI: 10.4251/wjgo.v15.i7.1227. PMID/PMCID: 37546562; PMC10401461.