Separation and Characterization of Heterogeneity Among Various Sizes of Outer Membrane Vesicles Derived from the Probiotic Escherichia coli Nissle 1917
Quick Facts
- Publication title: Separation and Characterization of Heterogeneity Among Various Sizes of Outer Membrane Vesicles Derived from the Probiotic Escherichia coli Nissle 1917
- Journal: Membranes (Basel)
- Year: 2025
- DOI: 10.3390/membranes15050141
- PMID/PMCID: 40422751; PMC12113494
Research overview
To address this knowledge gap, we developed a membrane filtration method to isolate EcN-derived OMVs of varying sizes. OMVs contain various biologically active substances from their parent bacteria, such as proteins, lipids, and nucleic acids.
Key findings
Outer membrane vesicles (OMVs) are extracellular vesicles secreted by Gram-negative bacteria with diameters of 20-250 nm.
OMVs contain various biologically active substances from their parent bacteria, such as proteins, lipids, and nucleic acids.
Escherichia coli Nissle 1917 (EcN) is a Gram-negative probiotic that resides in the human intestine.
EcN-derived OMVs are pivotal in modulating intestinal immune responses.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Separation and Characterization of Heterogeneity Among Various Sizes of Outer Membrane Vesicles Derived from the Probiotic Escherichia coli Nissle 1917 Membranes. 2025. DOI: 10.3390/membranes15050141. PMID/PMCID: 40422751; PMC12113494.