The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer

Quick Facts

  • Publication title: The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer
  • Journal: J Adv Res
  • Year: 2025
  • DOI: 10.1016/j.jare.2025.09.056
  • PMID/PMCID: 41043659; PMC13227286

Research overview

This study aims to establish and optimize an EV DNA methylation detection system and evaluate its diagnostic and classification potential for PCa. This study highlights the potential of urine EV DNA methylation as a novel diagnostic marker for PCa and lays a foundation for future EV DNA research.

Key findings

Heterogeneous biological characteristics were observed among DNA from different subtypes of EV, but methylation profiles remained consistent across subtypes and post-DNase I treatment.

EV DNA accurately reflected the methylation state of source cell genomic DNA.

By combining our screening results with data from the TCGA database and previously reported, we developed a panel consisting of 667 PCa-specific methylation targets for detection.

The diagnostic value of these six markers was further confirmed using methylight PCR in the validation cohort which also displayed promising performance as a tool for diagnosing PCa.

Study design

We characterized EV DNA biological properties, optimized pretreatment strategies, validated its correlation with genomic DNA methylation, and explored urine EV DNA methylation targets in 86 benign prostatic hyperplasia (BPH) and 109 PCa patients across three cohorts (screening: 30 BPH/33 PCa; training: 27 BPH/30 PCa; validation: 29 BPH/46 PCa).

Echo Biotech Role

Echo Biotech contributed EV isolation and purification, EV characterization.

Related platforms: Exoomics®

Related services and capabilities: Biofluid EV Isolation & Purification, Tissue EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB)

References

Original publication: The significance of urine extracellular vesicle DNA methylation detection in the diagnosis and classification of prostate cancer Journal of advanced research. 2025. DOI: 10.1016/j.jare.2025.09.056. PMID/PMCID: 41043659; PMC13227286.