Simultaneous Detection of Exosomal Membrane Protein and RNA by Highly Sensitive Aptamer Assisted Multiplex–PCR
Quick Facts
- Publication title: Simultaneous Detection of Exosomal Membrane Protein and RNA by Highly Sensitive Aptamer Assisted Multiplex–PCR
- Journal: ACS Appl Bio Mater
- Year: 2020
- DOI: 10.1021/acsabm.9b00825
- PMID/PMCID: 35025388
Research overview
, proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy. Although exosome detection offers a valuable chance to understand disease status in a multidimensional manner, comprehensive analysis of different exosomal biomolecules with limited specimens remains a challenge.
Key findings
, proteins, RNA, DNA, and lipids), have emerged as important analytes in liquid biopsy.
Although exosome detection offers a valuable chance to understand disease status in a multidimensional manner, comprehensive analysis of different exosomal biomolecules with limited specimens remains a challenge.
Herein, we introduced an aptamer-based PCR (polymerase chain reaction) platform and realized highly sensitive detection of CD63 protein positive exosomes as low as 50 particles/μL.
More importantly, simultaneous analysis of exosomal RNA and surface protein is available using this method.
Echo Biotech Role
Echo Biotech's specific contribution should be verified from the full text or internal project record.
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References
Original publication: Simultaneous Detection of Exosomal Membrane Protein and RNA by Highly Sensitive Aptamer Assisted Multiplex–PCR ACS Appl Bio Mater. 2020. DOI: 10.1021/acsabm.9b00825. PMID/PMCID: 35025388.