Small extracellular vesicle microRNAs in pediatric myasthenia gravis plasma and skeletal muscle
Quick Facts
- Publication title: Small extracellular vesicle microRNAs in pediatric myasthenia gravis plasma and skeletal muscle
- Journal: Postgrad Med J
- Year: 2024
- DOI: 10.1093/postmj/qgae015
- PMID/PMCID: 38449066
Research overview
In the present study, we used sEV isolation and purification methods to extract the plasma-derived sEV-miRNAs from children with MG and healthy controls. For the first time, we report that plasma-derived sEV-miRNAs may act as novel circulating biomarkers and therapeutic targets in pediatric MG.
Key findings
Twenty-four sEV-miRNAs with altered expression level were identified between groups by DESeq2 method.
The miRNAs were extracted from the sEVs, which were isolated from human primary skeletal muscle cell culture treated with mAb198.
The target genes and enriched pathways of sEV-miRNAs partially overlapped between cell supernatant and plasma samples.
The significantly downregulated miR-143-3p was validated in quantitative real-time polymerase chain reaction analysis.
Study design
In the present study, we used sEV isolation and purification methods to extract the plasma-derived sEV-miRNAs from children with MG and healthy controls.
A small RNA sequencing analysis confirmed the miRNA expression features in plasma-derived sEVs from MG patients.
The miRNA expression analysis in vitro was determined using microarray analysis.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, miRNA profiling/sequencing and bioinformatic analysis, qPCR validation; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Biofluid EV Isolation & Purification, Cell-Culture Supernatant EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV miRNA Profiling / Sequencing, qPCR Validation, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Small extracellular vesicle microRNAs in pediatric myasthenia gravis plasma and skeletal muscle Postgrad Med J. 2024. DOI: 10.1093/postmj/qgae015. PMID/PMCID: 38449066.