Synovial tissue-derived extracellular vesicles induce chondrocyte inflammation and degradation via NF-κB signalling pathway: An in vitro study

Quick Facts

  • Publication title: Synovial tissue-derived extracellular vesicles induce chondrocyte inflammation and degradation via NF-κB signalling pathway: An in vitro study
  • Journal: J Cell Mol Med
  • Year: 2022
  • DOI: 10.1111/jcmm.17227
  • PMID/PMCID: 35179308; PMC8980928

Research overview

Osteoarthritis (OA) is a whole-joint disease characterized by synovial inflammation and cartilage degeneration. However, the relationship between synovial inflammation and cartilage degeneration remains unclear.

Key findings

Osteoarthritis (OA) is a whole-joint disease characterized by synovial inflammation and cartilage degeneration.

However, the relationship between synovial inflammation and cartilage degeneration remains unclear.

The modified Hulth's method was adopted to establish a knee OA (KOA) rabbit model.

Synovial tissue was collected after 8 weeks, and synovial tissue-derived extracellular vesicles (ST-EVs) were extracted by filtration combined with size exclusion chromatography (SECF), followed by identification through transmission electron microscopy (TEM), nanoparticle tracer analysis (NTA) and Western blot (WB).

Echo Biotech Role

Echo Biotech contributed EV isolation and purification; the study also used or cited Exosupur®.

Related platforms: Exoomics®, Research Reagents & Tools

Related services and capabilities: EV Isolation & Purification, Research Reagent / Product Supply

Related products or reagents: Exosupur® EV Isolation/Purification Kit

References

Original publication: Synovial tissue-derived extracellular vesicles induce chondrocyte inflammation and degradation via NF-κB signalling pathway: An in vitro study J Cell Mol Med. 2022. DOI: 10.1111/jcmm.17227. PMID/PMCID: 35179308; PMC8980928.