Tetrastigma hemsleyanum alleviates febrile seizures by inhibiting HSPB1/Akt/NF-κB signaling via small extracellular vesicle-mediated astrocyte-microglia crosstalk
Quick Facts
- Publication title: Tetrastigma hemsleyanum alleviates febrile seizures by inhibiting HSPB1/Akt/NF-κB signaling via small extracellular vesicle-mediated astrocyte-microglia crosstalk
- Journal: Phytomedicine
- Year: 2026
- DOI: 10.1016/j.phymed.2026.158087
- PMID/PMCID: 41886953
Research overview
We found pretreatment with total flavonoids from T. Our results suggest FTH may alleviate FS by inhibiting the hippocampal HSPB1/Akt/NF-κB signaling pathway, which might initially be triggered by the release of sEVs from astrocytes to microglia.
Key findings
We found pretreatment with total flavonoids from T.
hemsleyanum (FTH) containing 36 components could inhibit FS in rats.
Microglia and astrocytes emerged as the two most prominent cell populations exhibiting significantly elevated heat shock protein B1 (Hspb1) gene expression after FS in the scRNA-seq data.
The inflammation-related microglial E subset was enriched in the FS model (MD) group and was associated with the response to heat and the MAPK signaling pathway.
Study design
We found pretreatment with total flavonoids from T.
hemsleyanum (FTH) containing 36 components could inhibit FS in rats.
Microglia and astrocytes emerged as the two most prominent cell populations exhibiting significantly elevated heat shock protein B1 (Hspb1) gene expression after FS in the scRNA-seq data.
Echo Biotech Role
Echo Biotech contributed EV isolation and purification, EV characterization, lncRNA/mRNA sequencing, EV proteomics, integrated multi-omics analysis; the study also used or cited Exosupur®.
Related platforms: Exoomics®, Research Reagents & Tools
Related services and capabilities: Tissue EV Isolation & Purification, Cell-Culture Supernatant EV Isolation & Purification, EV Basic Characterization (TEM/NTA/WB), EV lncRNA/mRNA Sequencing, EV Proteomics, Integrated Multi-omics Analysis, Research Reagent / Product Supply
Related products or reagents: Exosupur® EV Isolation/Purification Kit
References
Original publication: Tetrastigma hemsleyanum alleviates febrile seizures by inhibiting HSPB1/Akt/NF-κB signaling via small extracellular vesicle-mediated astrocyte-microglia crosstalk Phytomedicine. 2026. DOI: 10.1016/j.phymed.2026.158087. PMID/PMCID: 41886953.