Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease

Quick Facts

  • Publication title: Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease
  • Journal: CNS Neurosci Ther
  • Year: 2023
  • DOI: 10.1111/cns.14435
  • PMID/PMCID: 37664885; PMC10916443

Research overview

We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next-generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction. This study suggested that hsa-SCMH1_0001 and its target genes ARID1A and C1orf115 are downregulated in PD patients and may be involved in the occurrence of PD.

Key findings

According to the NGS, 62 upregulated and 37 downregulated circRNAs in the PD group were screened out.

Correlation analysis revealed that hsa-SCMH1_0001 has strong clinical relevance.

We identified 17 potential binding miRNAs of hsa-SCMH1_0001 with 149 potential target genes.

ARID1A and C1orf115 belong to the intersection of the predicted target genes and the differentially expressed mRNAs obtained by sequencing.

Study design

We examined circRNA and mRNA expression profiles in peripheral exosomes from PD patients (n = 23) and healthy controls (n = 15) using next-generation sequencing (NGS) technology, functional annotation, and quantitative polymerase chain reaction.

Correlation analysis was performed between the expression levels of the circRNAs and the clinical characteristics of PD patients.

The binding miRNAs and target genes were predicted using TargetScanHuman, miRDB, and miRTarBase.

Echo Biotech Role

Echo Biotech contributed lncRNA/mRNA sequencing.

Related platforms: Exoomics®

Related services and capabilities: EV lncRNA/mRNA Sequencing

References

Original publication: Transcriptome sequencing of circular RNA reveals the involvement of hsa-SCMH1_0001 in the pathogenesis of Parkinson's disease CNS Neurosci Ther. 2023. DOI: 10.1111/cns.14435. PMID/PMCID: 37664885; PMC10916443.